Derivation of two iPSC lines from a sporadic ASD patient (NUIGi033-A) and a paternal control (NUIGi034-A)

Berta Marcó de la Cruz; Yicheng Ding; Veronica McInerney; Janusz Krawczyk; Yin Lu; Guangming Yang; Xiaohong Qian; Weidong Li; Linda Howard; Nicholas M. Allen; Timothy O'Brien; Louise Gallagher; Sanbing Shen

doi:10.1016/j.scr.2020.101722

Derivation of two iPSC lines from a sporadic ASD patient (NUIGi033-A) and a paternal control (NUIGi034-A)

Berta Marcó de la Cruz
, Yicheng Ding
, Veronica McInerney
, Janusz Krawczyk
, Yin Lu
, Guangming Yang
, Xiaohong Qian
, Weidong Li
, Linda Howard
, Nicholas M. Allen
, Timothy O'Brien
, Louise Gallagher
, Sanbing Shen^*

^*Corresponding author for this work

University of Galway
Nanjing University of Chinese Medicine
Beijing Institute of Lifeomics
Shanghai Jiao Tong University
Our Lady's Hospital for Sick Children
Trinity College Dublin
Royal College of Surgeons in Ireland

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Hundreds of rare risk factors have been identified for ASD, however, the underlying causes for ~70% of sporadic cases are unknown. Sporadic ASD models are thus essential for validating phenotypic commonality and drug suitability to the majority of patients. Here, we derived induced pluripotent stem cells (iPSCs) from one sporadic ASD child and one paternal control, using non-integrating Sendai viral methods. The iPSCs strongly expressed pluripotency markers and could be differentiated into three germ layers. Their normal karyotype was validated by genome SNP array. The availability of sporadic ASD-derived iPSCs offers an opportunity for phenotypic comparison with genetic ASD models.

Original language	English
Article number	101722
Journal	Stem Cell Research
Volume	44
DOIs	https://doi.org/10.1016/j.scr.2020.101722
Publication status	Published - Apr 2020
Externally published	Yes

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title = "Derivation of two iPSC lines from a sporadic ASD patient (NUIGi033-A) and a paternal control (NUIGi034-A)",

abstract = "Hundreds of rare risk factors have been identified for ASD, however, the underlying causes for \textasciitilde{}70\% of sporadic cases are unknown. Sporadic ASD models are thus essential for validating phenotypic commonality and drug suitability to the majority of patients. Here, we derived induced pluripotent stem cells (iPSCs) from one sporadic ASD child and one paternal control, using non-integrating Sendai viral methods. The iPSCs strongly expressed pluripotency markers and could be differentiated into three germ layers. Their normal karyotype was validated by genome SNP array. The availability of sporadic ASD-derived iPSCs offers an opportunity for phenotypic comparison with genetic ASD models.",

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note = "Publisher Copyright: {\textcopyright} 2020 The Authors",

year = "2020",

month = apr,

doi = "10.1016/j.scr.2020.101722",

language = "English",

volume = "44",

journal = "Stem Cell Research",

issn = "1873-5061",

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TY - JOUR

T1 - Derivation of two iPSC lines from a sporadic ASD patient (NUIGi033-A) and a paternal control (NUIGi034-A)

AU - de la Cruz, Berta Marcó

AU - Ding, Yicheng

AU - McInerney, Veronica

AU - Krawczyk, Janusz

AU - Lu, Yin

AU - Yang, Guangming

AU - Qian, Xiaohong

AU - Li, Weidong

AU - Howard, Linda

AU - Allen, Nicholas M.

AU - O'Brien, Timothy

AU - Gallagher, Louise

AU - Shen, Sanbing

PY - 2020/4

Y1 - 2020/4

N2 - Hundreds of rare risk factors have been identified for ASD, however, the underlying causes for ~70% of sporadic cases are unknown. Sporadic ASD models are thus essential for validating phenotypic commonality and drug suitability to the majority of patients. Here, we derived induced pluripotent stem cells (iPSCs) from one sporadic ASD child and one paternal control, using non-integrating Sendai viral methods. The iPSCs strongly expressed pluripotency markers and could be differentiated into three germ layers. Their normal karyotype was validated by genome SNP array. The availability of sporadic ASD-derived iPSCs offers an opportunity for phenotypic comparison with genetic ASD models.

AB - Hundreds of rare risk factors have been identified for ASD, however, the underlying causes for ~70% of sporadic cases are unknown. Sporadic ASD models are thus essential for validating phenotypic commonality and drug suitability to the majority of patients. Here, we derived induced pluripotent stem cells (iPSCs) from one sporadic ASD child and one paternal control, using non-integrating Sendai viral methods. The iPSCs strongly expressed pluripotency markers and could be differentiated into three germ layers. Their normal karyotype was validated by genome SNP array. The availability of sporadic ASD-derived iPSCs offers an opportunity for phenotypic comparison with genetic ASD models.

UR - https://www.scopus.com/pages/publications/85079841651

U2 - 10.1016/j.scr.2020.101722

DO - 10.1016/j.scr.2020.101722

M3 - Article

C2 - 32097875

AN - SCOPUS:85079841651

SN - 1873-5061

VL - 44

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 101722

ER -

Derivation of two iPSC lines from a sporadic ASD patient (NUIGi033-A) and a paternal control (NUIGi034-A)

Abstract

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