Derivation of iPSC lines from two patients with autism spectrum disorder carrying NRXN1α deletion (NUIGi041-A, NUIG041-B; NUIGi045-A) and one sibling control (NUIGi042-A, NUIGi042-B)

Yicheng Ding; Aisling O'Brien; Berta Marcó de la Cruz; Meimei Yang; Jacqueline Fitzgerald; Guangming Yang; Weidong Li; Veronica McInerney; Janusz Krawczyk; Sally A. Lynch; Linda Howard; Nicholas M. Allen; Timothy O'Brien; Louise Gallagher; Sanbing Shen

doi:10.1016/j.scr.2021.102222

Derivation of iPSC lines from two patients with autism spectrum disorder carrying NRXN1α deletion (NUIGi041-A, NUIG041-B; NUIGi045-A) and one sibling control (NUIGi042-A, NUIGi042-B)

Yicheng Ding
, Aisling O'Brien
, Berta Marcó de la Cruz
, Meimei Yang
, Jacqueline Fitzgerald
, Guangming Yang
, Weidong Li
, Veronica McInerney
, Janusz Krawczyk
, Sally A. Lynch
, Linda Howard
, Nicholas M. Allen
, Timothy O'Brien
, Louise Gallagher^*
, Sanbing Shen

^*Corresponding author for this work

University of Galway
Royal College of Surgeons in Ireland
Trinity College Dublin
Nanjing University of Chinese Medicine
Shanghai Jiao Tong University
University College Dublin
Children’s Health Ireland

Research output: Contribution to journal › Article › peer-review

Abstract

NRXN1 encodes thousands of splicing variants categorized into long NRXN1α, short NRXN1β and extremely short NRXN1γ, which exert differential roles in neuronal excitation/inhibition. NRXN1α deletions are common in autism spectrum disorder (ASD) and other neurodevelopmental/neuropsychiatric disorders. We derived induced pluripotent stem cells (iPSCs) from one sibling control and two ASD probands carrying NRXN1α^+/−, using non-integrating Sendai viral method. All iPSCs highly expressed pluripotency markers and could be differentiated into ectodermal/mesodermal/endodermal cells. The genotype and karyotype of the iPSCs were validated by whole genome SNP array. The availability of the iPSCs offers an opportunity for understanding NRXN1α function in human neurons and in ASD.

Original language	English
Article number	102222
Journal	Stem Cell Research
Volume	52
DOIs	https://doi.org/10.1016/j.scr.2021.102222
Publication status	Published - Apr 2021
Externally published	Yes

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Ding, Y., O'Brien, A., de la Cruz, B. M., Yang, M., Fitzgerald, J., Yang, G., Li, W., McInerney, V., Krawczyk, J., Lynch, S. A., Howard, L., Allen, N. M., O'Brien, T., Gallagher, L., & Shen, S. (2021). Derivation of iPSC lines from two patients with autism spectrum disorder carrying NRXN1α deletion (NUIGi041-A, NUIG041-B; NUIGi045-A) and one sibling control (NUIGi042-A, NUIGi042-B). Stem Cell Research, 52, Article 102222. https://doi.org/10.1016/j.scr.2021.102222

@article{31e62494b5ed467eac7a49d5a1842d83,

title = "Derivation of iPSC lines from two patients with autism spectrum disorder carrying NRXN1α deletion (NUIGi041-A, NUIG041-B; NUIGi045-A) and one sibling control (NUIGi042-A, NUIGi042-B)",

abstract = "NRXN1 encodes thousands of splicing variants categorized into long NRXN1α, short NRXN1β and extremely short NRXN1γ, which exert differential roles in neuronal excitation/inhibition. NRXN1α deletions are common in autism spectrum disorder (ASD) and other neurodevelopmental/neuropsychiatric disorders. We derived induced pluripotent stem cells (iPSCs) from one sibling control and two ASD probands carrying NRXN1α+/−, using non-integrating Sendai viral method. All iPSCs highly expressed pluripotency markers and could be differentiated into ectodermal/mesodermal/endodermal cells. The genotype and karyotype of the iPSCs were validated by whole genome SNP array. The availability of the iPSCs offers an opportunity for understanding NRXN1α function in human neurons and in ASD.",

author = "Yicheng Ding and Aisling O'Brien and \{de la Cruz\}, \{Berta Marc{\'o}\} and Meimei Yang and Jacqueline Fitzgerald and Guangming Yang and Weidong Li and Veronica McInerney and Janusz Krawczyk and Lynch, \{Sally A.\} and Linda Howard and Allen, \{Nicholas M.\} and Timothy O'Brien and Louise Gallagher and Sanbing Shen",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

month = apr,

doi = "10.1016/j.scr.2021.102222",

language = "English",

volume = "52",

journal = "Stem Cell Research",

issn = "1873-5061",

}

Ding, Y, O'Brien, A, de la Cruz, BM, Yang, M, Fitzgerald, J, Yang, G, Li, W, McInerney, V, Krawczyk, J, Lynch, SA, Howard, L, Allen, NM, O'Brien, T, Gallagher, L & Shen, S 2021, 'Derivation of iPSC lines from two patients with autism spectrum disorder carrying NRXN1α deletion (NUIGi041-A, NUIG041-B; NUIGi045-A) and one sibling control (NUIGi042-A, NUIGi042-B)', Stem Cell Research, vol. 52, 102222. https://doi.org/10.1016/j.scr.2021.102222

TY - JOUR

T1 - Derivation of iPSC lines from two patients with autism spectrum disorder carrying NRXN1α deletion (NUIGi041-A, NUIG041-B; NUIGi045-A) and one sibling control (NUIGi042-A, NUIGi042-B)

AU - Ding, Yicheng

AU - O'Brien, Aisling

AU - de la Cruz, Berta Marcó

AU - Yang, Meimei

AU - Fitzgerald, Jacqueline

AU - Yang, Guangming

AU - Li, Weidong

AU - McInerney, Veronica

AU - Krawczyk, Janusz

AU - Lynch, Sally A.

AU - Howard, Linda

AU - Allen, Nicholas M.

AU - O'Brien, Timothy

AU - Gallagher, Louise

AU - Shen, Sanbing

PY - 2021/4

Y1 - 2021/4

N2 - NRXN1 encodes thousands of splicing variants categorized into long NRXN1α, short NRXN1β and extremely short NRXN1γ, which exert differential roles in neuronal excitation/inhibition. NRXN1α deletions are common in autism spectrum disorder (ASD) and other neurodevelopmental/neuropsychiatric disorders. We derived induced pluripotent stem cells (iPSCs) from one sibling control and two ASD probands carrying NRXN1α+/−, using non-integrating Sendai viral method. All iPSCs highly expressed pluripotency markers and could be differentiated into ectodermal/mesodermal/endodermal cells. The genotype and karyotype of the iPSCs were validated by whole genome SNP array. The availability of the iPSCs offers an opportunity for understanding NRXN1α function in human neurons and in ASD.

AB - NRXN1 encodes thousands of splicing variants categorized into long NRXN1α, short NRXN1β and extremely short NRXN1γ, which exert differential roles in neuronal excitation/inhibition. NRXN1α deletions are common in autism spectrum disorder (ASD) and other neurodevelopmental/neuropsychiatric disorders. We derived induced pluripotent stem cells (iPSCs) from one sibling control and two ASD probands carrying NRXN1α+/−, using non-integrating Sendai viral method. All iPSCs highly expressed pluripotency markers and could be differentiated into ectodermal/mesodermal/endodermal cells. The genotype and karyotype of the iPSCs were validated by whole genome SNP array. The availability of the iPSCs offers an opportunity for understanding NRXN1α function in human neurons and in ASD.

UR - https://www.scopus.com/pages/publications/85100633618

U2 - 10.1016/j.scr.2021.102222

DO - 10.1016/j.scr.2021.102222

M3 - Article

C2 - 33578364

AN - SCOPUS:85100633618

SN - 1873-5061

VL - 52

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 102222

ER -

Derivation of iPSC lines from two patients with autism spectrum disorder carrying NRXN1α deletion (NUIGi041-A, NUIG041-B; NUIGi045-A) and one sibling control (NUIGi042-A, NUIGi042-B)

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