TY - JOUR
T1 - Dendritic cell surface calreticulin is a receptor for NY-ESO-1
T2 - Direct interactions between tumor-associated antigen and the innate immune system
AU - Zeng, Gang
AU - Aldridge, Michael E.
AU - Tian, Xiaoli
AU - Seiler, Daniel
AU - Zhang, Xiaolong
AU - Jin, Yusheng
AU - Rao, Jianyu
AU - Li, Weidong
AU - Chen, Dequan
AU - Langford, Marlyn P.
AU - Duggan, Chris
AU - Belldegrun, Arie S.
AU - Dubinett, Steven M.
PY - 2006/9/15
Y1 - 2006/9/15
N2 - How the immune system recognizes endogenously arising tumors and elicits adaptive immune responses against nonmutated tumor-associated Ags is poorly understood. In search of intrinsic factors contributing to the immunogenicity of the tumor-associated Ag NY-ESO-1, we found that the NY-ESO-1 protein binds to the surface of immature dendritic cells (DC), macrophages, and monocytes, but not to that of B cells or T cells. Using immunoprecipitation coupled with tandem mass spectrometry, we isolated DC surface calreticulin as the receptor for NY-ESO-1. Calreticulin Abs blocked NY-ESO-1 binding on immature DC and its cross-presentation to CD8+ T cells in vitro. Calreticulin/NY-ESO-1 interactions provide a direct link between NY-ESO-1, the innate immune system, and, potentially, the adaptive immune response against NY-ESO-1.
AB - How the immune system recognizes endogenously arising tumors and elicits adaptive immune responses against nonmutated tumor-associated Ags is poorly understood. In search of intrinsic factors contributing to the immunogenicity of the tumor-associated Ag NY-ESO-1, we found that the NY-ESO-1 protein binds to the surface of immature dendritic cells (DC), macrophages, and monocytes, but not to that of B cells or T cells. Using immunoprecipitation coupled with tandem mass spectrometry, we isolated DC surface calreticulin as the receptor for NY-ESO-1. Calreticulin Abs blocked NY-ESO-1 binding on immature DC and its cross-presentation to CD8+ T cells in vitro. Calreticulin/NY-ESO-1 interactions provide a direct link between NY-ESO-1, the innate immune system, and, potentially, the adaptive immune response against NY-ESO-1.
UR - https://www.scopus.com/pages/publications/33748489532
U2 - 10.4049/jimmunol.177.6.3582
DO - 10.4049/jimmunol.177.6.3582
M3 - Article
C2 - 16951317
AN - SCOPUS:33748489532
SN - 0022-1767
VL - 177
SP - 3582
EP - 3589
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -