TY - JOUR
T1 - A screen-printed, amperometric biosensor for the determination of organophosphorus pesticides in water samples
AU - Dou, Junfeng
AU - Ding, Aizhong
AU - Cheng, Lirong
AU - Sekar, Raju
AU - Wang, Hongting
AU - Li, Shuairan
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (No. 51008026, 40873076 and 41011130204).
PY - 2012/5
Y1 - 2012/5
N2 - An amperometric biosensor based on screen-printed electrodes (SPEs) was developed for the determination of organophosphorus pesticides in water samples. The extent of acetylcholinesterase (AChE) deactivation was determined and quantified for pesticide concentrations in water samples. An enzyme immobilization adsorption procedure and polyacrylamide gel matrix polymerization were used for fabrication of the biosensor, with minimal losses in enzyme activity. The optimal conditions for enzyme catalytic reaction on the SPEs surfaces were acetylthiocholine chloride (ATChCl) concentration of 5 mmol/L, pH 7 and reaction time of 4 min. The detection limits for three organophosphorus pesticides (dichlorvos, monocrotophs and parathion) were in the range of 4 to 7 μg/L when an AChE amount of 0.1 U was used for immobilization.
AB - An amperometric biosensor based on screen-printed electrodes (SPEs) was developed for the determination of organophosphorus pesticides in water samples. The extent of acetylcholinesterase (AChE) deactivation was determined and quantified for pesticide concentrations in water samples. An enzyme immobilization adsorption procedure and polyacrylamide gel matrix polymerization were used for fabrication of the biosensor, with minimal losses in enzyme activity. The optimal conditions for enzyme catalytic reaction on the SPEs surfaces were acetylthiocholine chloride (ATChCl) concentration of 5 mmol/L, pH 7 and reaction time of 4 min. The detection limits for three organophosphorus pesticides (dichlorvos, monocrotophs and parathion) were in the range of 4 to 7 μg/L when an AChE amount of 0.1 U was used for immobilization.
KW - Acetylcholinesterase
KW - Biosensor
KW - Enzyme immobilization
KW - Organophosphorus pesticides
KW - Screen-printed electrodes
UR - https://www.scopus.com/pages/publications/84860848809
U2 - 10.1016/S1001-0742(11)60864-4
DO - 10.1016/S1001-0742(11)60864-4
M3 - Article
C2 - 22893976
AN - SCOPUS:84860848809
SN - 1001-0742
VL - 24
SP - 956
EP - 962
JO - Journal of Environmental Sciences (China)
JF - Journal of Environmental Sciences (China)
IS - 5
ER -