A Paper-Based Microfluidic POCT Device for Automatic Recombinase Polymerase Amplification Detection

Weiqin. Xu; Jihong Sun; Tong Li; Pengfei Song

A Paper-Based Microfluidic POCT Device for Automatic Recombinase Polymerase Amplification Detection

Weiqin. Xu
, Jihong Sun
, Tong Li
, Pengfei Song^*

^*Corresponding author for this work

Xi'an Jiaotong-Liverpool University

Research output: Chapter in Book or Report/Conference proceeding › Conference Proceeding › peer-review

Abstract

This paper presents a fully automated microfluidic paper-based device for rapid
detection of African swine fever virus (ASFV) and pseudorabies virus (PRV) using recombinase
polymerase amplification (RPA). To address limitations of conventional paper-based RPA
systems—including slow reagent transport due to viscous polymer additives (e.g., PEG) and
manual intervention—we developed a self-contained μPAD featuring a programmable rotary
valve and surface-modified channels. Oxygen plasma treatment combined with PVP
(Polyvinylpyrrolidone) coating accelerated capillary flow by 64% (transfer time: 25 s vs. >80 s),
while on-chip temperature control (42°C) and fluorescence detection enabled complete "samplein, answer-out" operation. The device achieved detection limits of 5 copies/μL (ASFV) and 10 copies/μL (PRV) within 12–16 minutes, demonstrating a portable, instrument-free solution for
point-of-care viral screening.

Original language	English
Title of host publication	Journal of Physics: Conference Series
Publication status	Published - 1 Sept 2025

Cite this

@inproceedings{593663cbbc324677a4d9561c92808212,

title = "A Paper-Based Microfluidic POCT Device for Automatic Recombinase Polymerase Amplification Detection",

abstract = "This paper presents a fully automated microfluidic paper-based device for rapid detection of African swine fever virus (ASFV) and pseudorabies virus (PRV) using recombinase polymerase amplification (RPA). To address limitations of conventional paper-based RPA systems—including slow reagent transport due to viscous polymer additives (e.g., PEG) and manual intervention—we developed a self-contained μPAD featuring a programmable rotary valve and surface-modified channels. Oxygen plasma treatment combined with PVP (Polyvinylpyrrolidone) coating accelerated capillary flow by 64\% (transfer time: 25 s vs. >80 s), while on-chip temperature control (42°C) and fluorescence detection enabled complete {"}samplein, answer-out{"} operation. The device achieved detection limits of 5 copies/μL (ASFV) and 10 copies/μL (PRV) within 12–16 minutes, demonstrating a portable, instrument-free solution for point-of-care viral screening.",

author = "Weiqin. Xu and Jihong Sun and Tong Li and Pengfei Song",

year = "2025",

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T1 - A Paper-Based Microfluidic POCT Device for Automatic Recombinase Polymerase Amplification Detection

AU - Xu, Weiqin.

AU - Sun, Jihong

AU - Li, Tong

AU - Song, Pengfei

PY - 2025/9/1

Y1 - 2025/9/1

N2 - This paper presents a fully automated microfluidic paper-based device for rapid detection of African swine fever virus (ASFV) and pseudorabies virus (PRV) using recombinase polymerase amplification (RPA). To address limitations of conventional paper-based RPA systems—including slow reagent transport due to viscous polymer additives (e.g., PEG) and manual intervention—we developed a self-contained μPAD featuring a programmable rotary valve and surface-modified channels. Oxygen plasma treatment combined with PVP (Polyvinylpyrrolidone) coating accelerated capillary flow by 64% (transfer time: 25 s vs. >80 s), while on-chip temperature control (42°C) and fluorescence detection enabled complete "samplein, answer-out" operation. The device achieved detection limits of 5 copies/μL (ASFV) and 10 copies/μL (PRV) within 12–16 minutes, demonstrating a portable, instrument-free solution for point-of-care viral screening.

AB - This paper presents a fully automated microfluidic paper-based device for rapid detection of African swine fever virus (ASFV) and pseudorabies virus (PRV) using recombinase polymerase amplification (RPA). To address limitations of conventional paper-based RPA systems—including slow reagent transport due to viscous polymer additives (e.g., PEG) and manual intervention—we developed a self-contained μPAD featuring a programmable rotary valve and surface-modified channels. Oxygen plasma treatment combined with PVP (Polyvinylpyrrolidone) coating accelerated capillary flow by 64% (transfer time: 25 s vs. >80 s), while on-chip temperature control (42°C) and fluorescence detection enabled complete "samplein, answer-out" operation. The device achieved detection limits of 5 copies/μL (ASFV) and 10 copies/μL (PRV) within 12–16 minutes, demonstrating a portable, instrument-free solution for point-of-care viral screening.

M3 - Conference Proceeding

BT - Journal of Physics: Conference Series

ER -

A Paper-Based Microfluidic POCT Device for Automatic Recombinase Polymerase Amplification Detection

Abstract

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