A Paper-Based Microfluidic Analytical Device with A Highly Integrated On-Chip Valve for Autonomous ELISA

Tianyu Cai; Sixuan Duan; Hao Fu; Jia Zhu; Eng Gee Lim; Kaizhu Huang; Kai Hoettges; Xinyu Liu; Pengfei Song

doi:10.1109/MEMS51670.2022.9699652

A Paper-Based Microfluidic Analytical Device with A Highly Integrated On-Chip Valve for Autonomous ELISA

Tianyu Cai
, Sixuan Duan
, Hao Fu
, Jia Zhu
, Eng Gee Lim
, Kaizhu Huang
, Kai Hoettges
, Xinyu Liu
, Pengfei Song

Xi'an Jiaotong-Liverpool University
University of Liverpool
Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
Suzhou City University
University of Toronto

Research output: Chapter in Book or Report/Conference proceeding › Conference Proceeding › peer-review

12 Citations (Scopus)

Abstract

This paper reports a microfluidic paper-based analytical device (PAD), featuring a novel, highly integrated on-chip rotary valve, for autonomous enzyme-linked immunosorbent assay (ELISA). Unlike existing valving methods on PADs, this rotary valve allows regulation of multiple flows with a single valve, leading to ease of assembly and small footprint with a high integration level. The micromotor-controlled valve is also programmable and highly reliable, and has a fast response (76 ± 0.6ms, n=20, 500 times faster than previous smart material-enabled valves on PADs). Using this design, we performed autonomous ELISA, demonstrating a limit of detection (LOD) of 20 pM for rabbit IgG antibody detection with 100% valving reliability.

Original language	English
Title of host publication	35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	271-274
Number of pages	4
ISBN (Electronic)	9781665409117
DOIs	https://doi.org/10.1109/MEMS51670.2022.9699652
Publication status	Published - 2022
Event	35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 - Tokyo, Japan Duration: 9 Jan 2022 → 13 Jan 2022

Conference

Conference	35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
Country/Territory	Japan
City	Tokyo
Period	9/01/22 → 13/01/22

Keywords

Microfluidic paper-based analytical device
autonomous ELISA
in-vitro diagnostics
on-chip valve

Access to Document

10.1109/MEMS51670.2022.9699652

Cite this

Cai, T., Duan, S., Fu, H., Zhu, J., Lim, E. G., Huang, K., Hoettges, K., Liu, X., & Song, P. (2022). A Paper-Based Microfluidic Analytical Device with A Highly Integrated On-Chip Valve for Autonomous ELISA. In 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 (pp. 271-274). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMS51670.2022.9699652

@inproceedings{1b8059e2bc854b74881360d468d15a34,

title = "A Paper-Based Microfluidic Analytical Device with A Highly Integrated On-Chip Valve for Autonomous ELISA",

abstract = "This paper reports a microfluidic paper-based analytical device (PAD), featuring a novel, highly integrated on-chip rotary valve, for autonomous enzyme-linked immunosorbent assay (ELISA). Unlike existing valving methods on PADs, this rotary valve allows regulation of multiple flows with a single valve, leading to ease of assembly and small footprint with a high integration level. The micromotor-controlled valve is also programmable and highly reliable, and has a fast response (76 ± 0.6ms, n=20, 500 times faster than previous smart material-enabled valves on PADs). Using this design, we performed autonomous ELISA, demonstrating a limit of detection (LOD) of 20 pM for rabbit IgG antibody detection with 100\% valving reliability.",

keywords = "Microfluidic paper-based analytical device, autonomous ELISA, in-vitro diagnostics, on-chip valve",

author = "Tianyu Cai and Sixuan Duan and Hao Fu and Jia Zhu and Lim, \{Eng Gee\} and Kaizhu Huang and Kai Hoettges and Xinyu Liu and Pengfei Song",

note = "Funding Information: T. Cai and S. Duan contributed equally to this work. The authors thank the financial support from the programs of 2020 Natural Science Foundation of the Jiangsu Higher Education (20KJB460024), Jiangsu Science and Technology Programme - Young Scholar (SBK2020041995), Jiangsu Province High-level Innovation and Entrepreneurship Talent Plan (2020-30803), XJTLU Key Programme Special Fund – Exploratory Research Programme (KSF-E-39), and XJTLU Research Development Fund (RDF-18-02-20). The authors also acknowledge the financial support from Xi{\textquoteright}an Jiaotong – Liverpool University to S. Duan (PGRS1912019). Publisher Copyright: {\textcopyright} 2022 IEEE.; 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 ; Conference date: 09-01-2022 Through 13-01-2022",

year = "2022",

doi = "10.1109/MEMS51670.2022.9699652",

language = "English",

pages = "271--274",

booktitle = "35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

}

Cai, T, Duan, S, Fu, H, Zhu, J, Lim, EG, Huang, K, Hoettges, K, Liu, X & Song, P 2022, A Paper-Based Microfluidic Analytical Device with A Highly Integrated On-Chip Valve for Autonomous ELISA. in 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022. Institute of Electrical and Electronics Engineers Inc., pp. 271-274, 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022, Tokyo, Japan, 9/01/22. https://doi.org/10.1109/MEMS51670.2022.9699652

A Paper-Based Microfluidic Analytical Device with A Highly Integrated On-Chip Valve for Autonomous ELISA. / Cai, Tianyu; Duan, Sixuan; Fu, Hao et al.
35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022. Institute of Electrical and Electronics Engineers Inc., 2022. p. 271-274.

Research output: Chapter in Book or Report/Conference proceeding › Conference Proceeding › peer-review

TY - GEN

T1 - A Paper-Based Microfluidic Analytical Device with A Highly Integrated On-Chip Valve for Autonomous ELISA

AU - Cai, Tianyu

AU - Duan, Sixuan

AU - Fu, Hao

AU - Zhu, Jia

AU - Lim, Eng Gee

AU - Huang, Kaizhu

AU - Hoettges, Kai

AU - Liu, Xinyu

AU - Song, Pengfei

N1 - Funding Information: T. Cai and S. Duan contributed equally to this work. The authors thank the financial support from the programs of 2020 Natural Science Foundation of the Jiangsu Higher Education (20KJB460024), Jiangsu Science and Technology Programme - Young Scholar (SBK2020041995), Jiangsu Province High-level Innovation and Entrepreneurship Talent Plan (2020-30803), XJTLU Key Programme Special Fund – Exploratory Research Programme (KSF-E-39), and XJTLU Research Development Fund (RDF-18-02-20). The authors also acknowledge the financial support from Xi’an Jiaotong – Liverpool University to S. Duan (PGRS1912019). Publisher Copyright: © 2022 IEEE.

PY - 2022

Y1 - 2022

N2 - This paper reports a microfluidic paper-based analytical device (PAD), featuring a novel, highly integrated on-chip rotary valve, for autonomous enzyme-linked immunosorbent assay (ELISA). Unlike existing valving methods on PADs, this rotary valve allows regulation of multiple flows with a single valve, leading to ease of assembly and small footprint with a high integration level. The micromotor-controlled valve is also programmable and highly reliable, and has a fast response (76 ± 0.6ms, n=20, 500 times faster than previous smart material-enabled valves on PADs). Using this design, we performed autonomous ELISA, demonstrating a limit of detection (LOD) of 20 pM for rabbit IgG antibody detection with 100% valving reliability.

AB - This paper reports a microfluidic paper-based analytical device (PAD), featuring a novel, highly integrated on-chip rotary valve, for autonomous enzyme-linked immunosorbent assay (ELISA). Unlike existing valving methods on PADs, this rotary valve allows regulation of multiple flows with a single valve, leading to ease of assembly and small footprint with a high integration level. The micromotor-controlled valve is also programmable and highly reliable, and has a fast response (76 ± 0.6ms, n=20, 500 times faster than previous smart material-enabled valves on PADs). Using this design, we performed autonomous ELISA, demonstrating a limit of detection (LOD) of 20 pM for rabbit IgG antibody detection with 100% valving reliability.

KW - Microfluidic paper-based analytical device

KW - autonomous ELISA

KW - in-vitro diagnostics

KW - on-chip valve

UR - https://www.scopus.com/pages/publications/85126394524

U2 - 10.1109/MEMS51670.2022.9699652

DO - 10.1109/MEMS51670.2022.9699652

M3 - Conference Proceeding

AN - SCOPUS:85126394524

SP - 271

EP - 274

BT - 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022

Y2 - 9 January 2022 through 13 January 2022

ER -

A Paper-Based Microfluidic Analytical Device with A Highly Integrated On-Chip Valve for Autonomous ELISA

Abstract

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Cite this