A Modified Miniscope System for Simultaneous Electrophysiology and Calcium Imaging in vivo

Xiaoting Wu; Xiangyu Yang; Lulu Song; Yang Wang; Yamin Li; Yuanyuan Liu; Xiaowei Yang; Yijun Wang; Weihua Pei; Weidong Li

doi:10.3389/fnint.2021.682019

A Modified Miniscope System for Simultaneous Electrophysiology and Calcium Imaging in vivo

Xiaoting Wu
, Xiangyu Yang
, Lulu Song
, Yang Wang
, Yamin Li
, Yuanyuan Liu
, Xiaowei Yang
, Yijun Wang
, Weihua Pei^*
, Weidong Li^*

^*Corresponding author for this work

CAS - Institute of Semiconductors
University of Chinese Academy of Sciences
Shanghai Jiao Tong University
Chinese Academy of Sciences

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

The miniscope system is one of the calcium (Ca²⁺) imaging tools with small size and lightweight and can realize the deep-brain Ca²⁺ imaging not confined to the cerebral cortex. Combining Ca²⁺ imaging and electrophysiology recording has been an efficient method for extracting high temporal-spatial resolution signals in the brain. In this study, a particular electrode probe was developed and assembled on the imaging lens to modify the miniscope system. The electrode probe can be tightly integrated into the lens of the miniscope without increasing the volume, weight, and implantation complexity. In vivo tests verified that the proposed modified system has realized the simultaneous recording of Ca²⁺ signals and local field potential (LFP) signal in the hippocampus CA1 region of an adult mouse.

Original language	English
Article number	682019
Journal	Frontiers in Integrative Neuroscience
Volume	15
DOIs	https://doi.org/10.3389/fnint.2021.682019
Publication status	Published - 16 Aug 2021
Externally published	Yes

Keywords

calcium imaging
electrophysiology recording
high resolution
modified miniscope system
probe

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10.3389/fnint.2021.682019

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title = "A Modified Miniscope System for Simultaneous Electrophysiology and Calcium Imaging in vivo",

abstract = "The miniscope system is one of the calcium (Ca2+) imaging tools with small size and lightweight and can realize the deep-brain Ca2+ imaging not confined to the cerebral cortex. Combining Ca2+ imaging and electrophysiology recording has been an efficient method for extracting high temporal-spatial resolution signals in the brain. In this study, a particular electrode probe was developed and assembled on the imaging lens to modify the miniscope system. The electrode probe can be tightly integrated into the lens of the miniscope without increasing the volume, weight, and implantation complexity. In vivo tests verified that the proposed modified system has realized the simultaneous recording of Ca2+ signals and local field potential (LFP) signal in the hippocampus CA1 region of an adult mouse.",

keywords = "calcium imaging, electrophysiology recording, high resolution, modified miniscope system, probe",

author = "Xiaoting Wu and Xiangyu Yang and Lulu Song and Yang Wang and Yamin Li and Yuanyuan Liu and Xiaowei Yang and Yijun Wang and Weihua Pei and Weidong Li",

note = "Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Wu, Yang, Song, Wang, Li, Liu, Yang, Wang, Pei and Li.",

year = "2021",

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day = "16",

doi = "10.3389/fnint.2021.682019",

language = "English",

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journal = "Frontiers in Integrative Neuroscience",

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TY - JOUR

T1 - A Modified Miniscope System for Simultaneous Electrophysiology and Calcium Imaging in vivo

AU - Wu, Xiaoting

AU - Yang, Xiangyu

AU - Song, Lulu

AU - Wang, Yang

AU - Li, Yamin

AU - Liu, Yuanyuan

AU - Yang, Xiaowei

AU - Wang, Yijun

AU - Pei, Weihua

AU - Li, Weidong

PY - 2021/8/16

Y1 - 2021/8/16

N2 - The miniscope system is one of the calcium (Ca2+) imaging tools with small size and lightweight and can realize the deep-brain Ca2+ imaging not confined to the cerebral cortex. Combining Ca2+ imaging and electrophysiology recording has been an efficient method for extracting high temporal-spatial resolution signals in the brain. In this study, a particular electrode probe was developed and assembled on the imaging lens to modify the miniscope system. The electrode probe can be tightly integrated into the lens of the miniscope without increasing the volume, weight, and implantation complexity. In vivo tests verified that the proposed modified system has realized the simultaneous recording of Ca2+ signals and local field potential (LFP) signal in the hippocampus CA1 region of an adult mouse.

AB - The miniscope system is one of the calcium (Ca2+) imaging tools with small size and lightweight and can realize the deep-brain Ca2+ imaging not confined to the cerebral cortex. Combining Ca2+ imaging and electrophysiology recording has been an efficient method for extracting high temporal-spatial resolution signals in the brain. In this study, a particular electrode probe was developed and assembled on the imaging lens to modify the miniscope system. The electrode probe can be tightly integrated into the lens of the miniscope without increasing the volume, weight, and implantation complexity. In vivo tests verified that the proposed modified system has realized the simultaneous recording of Ca2+ signals and local field potential (LFP) signal in the hippocampus CA1 region of an adult mouse.

KW - calcium imaging

KW - electrophysiology recording

KW - high resolution

KW - modified miniscope system

KW - probe

UR - https://www.scopus.com/pages/publications/85114299104

U2 - 10.3389/fnint.2021.682019

DO - 10.3389/fnint.2021.682019

M3 - Article

AN - SCOPUS:85114299104

SN - 1662-5145

VL - 15

JO - Frontiers in Integrative Neuroscience

JF - Frontiers in Integrative Neuroscience

M1 - 682019

ER -

A Modified Miniscope System for Simultaneous Electrophysiology and Calcium Imaging in vivo

Abstract

Keywords

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