TY - JOUR
T1 - A microfluidic field-effect transistor biosensor with rolled-up indium nitride microtubes
AU - Song, Pengfei
AU - Fu, Hao
AU - Wang, Yongjie
AU - Chen, Cheng
AU - Ou, Pengfei
AU - Rashid, Roksana Tonny
AU - Duan, Sixuan
AU - Song, Jun
AU - Mi, Zetian
AU - Liu, Xinyu
N1 - Funding Information:
This research was supported by Natural Sciences and Engineering Research Council of Canada (NSERC) (grant numbers: STPGP-2014-463182 , RGPIN-2017-06374 , and RGPAS-2017-507980 ), and the Canada Foundation for Innovation (grant number: JELF-37812 ). The financial support from Xi'an Jiaotong – Liverpool University to P. Song is also acknowledged (grant numbers: RDF-18-02-20, KSF-E-39 ). The authors also acknowledge the financial support from the University of Toronto to X. Liu (through the Percy Hart Edward Professorship) and the Chinese Scholarship Council to P. Song and C. Chen (through CSC scholarships). The authors also acknowledge the financial support from CMC Microsystems for microfabrication.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/10/15
Y1 - 2021/10/15
N2 - Field-effect-transistor (FET) biosensors capable of rapidly detecting disease-relevant biomarkers have long been considered as a promising tool for point-of-care (POC) diagnosis. Rolled-up nanotechnology, as a batch fabrication strategy for generating three-dimensional (3D) microtubes, has been demonstrated to possess unique advantages for constructing FET biosensors. In this paper, we report a new approach combining the two fascinating technologies, the FET biosensor and the rolled-up microtube, to develop a microfluidic diagnostic biosensor. We integrated an excellent biosensing III-nitride material—indium nitride (InN)—into a rolled-up microtube and used it as the FET channel. The InN possesses strong, intrinsic, and stable electron accumulation (~1013 cm−2) on its surface, thereby providing a high device sensitivity. Multiple rolled-up InN microtube FET biosensors fabricated on the same substrate were integrated with a microfluidic channel for convenient fluids handling, and shared the same external electrode (inserted into the microchannel outlet) for gating voltage modulation. Using human immunodeficiency virus (HIV) antibody as a model disease marker, we characterized the analytical performance of the developed biosensor and achieved a limit of detection (LOD) of 2.5 pM for serum samples spiked with HIV gp41 antibodies. The rolled-up InN microtube FET biosensor represents a new type of III-nitride-based FET biosensor and holds significant potential for practical POC diagnosis.
AB - Field-effect-transistor (FET) biosensors capable of rapidly detecting disease-relevant biomarkers have long been considered as a promising tool for point-of-care (POC) diagnosis. Rolled-up nanotechnology, as a batch fabrication strategy for generating three-dimensional (3D) microtubes, has been demonstrated to possess unique advantages for constructing FET biosensors. In this paper, we report a new approach combining the two fascinating technologies, the FET biosensor and the rolled-up microtube, to develop a microfluidic diagnostic biosensor. We integrated an excellent biosensing III-nitride material—indium nitride (InN)—into a rolled-up microtube and used it as the FET channel. The InN possesses strong, intrinsic, and stable electron accumulation (~1013 cm−2) on its surface, thereby providing a high device sensitivity. Multiple rolled-up InN microtube FET biosensors fabricated on the same substrate were integrated with a microfluidic channel for convenient fluids handling, and shared the same external electrode (inserted into the microchannel outlet) for gating voltage modulation. Using human immunodeficiency virus (HIV) antibody as a model disease marker, we characterized the analytical performance of the developed biosensor and achieved a limit of detection (LOD) of 2.5 pM for serum samples spiked with HIV gp41 antibodies. The rolled-up InN microtube FET biosensor represents a new type of III-nitride-based FET biosensor and holds significant potential for practical POC diagnosis.
KW - Biosensor
KW - Disease diagnostics
KW - Field-effect transistor
KW - Indium nitride
KW - Rolled-up microtube
UR - https://www.scopus.com/pages/publications/85109456406
U2 - 10.1016/j.bios.2021.113264
DO - 10.1016/j.bios.2021.113264
M3 - Article
C2 - 34225055
AN - SCOPUS:85109456406
SN - 0956-5663
VL - 190
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 113264
ER -