Activated macrophages have been shown to play an integral role in the etiology of diseases, such as cancer initiation and progression, lupus, ulcerative colitis, and diabetes et al. Many pattern recognition receptors are upregulated on activated macrophages, which leads to endocytosis. Endocytosis is a critical biological process for cell growth and viability. It promotes the transportation of macromolecules that cannot pass through the membrane passively. Endocytosis also plays important roles in the delivery of medicines for therapeutic treatments. Over the last decades, a lot of research has been focused on developing advanced imaging methods to monitor endocytosis events in living cells and tissues. We aim to use fluorescence methods to study endocytosis of proteins, directly monitoring the molecular machinery of endocytic pathways in macrophage cells. Through this study, students will be able to receive basic biochemical training, and to be exposed to frontier research in the field of targeted biomedicine delivery.