Pancreatic cancer (PDA) is an extremely lethal tumor with a death rate » 90% in 5 years. Most of pancreatic cancer patients are either unresectable or metastatic disease. Even some new diagnostic and treatment techniques are developed, the patient outcomes are still modest. In human, only ~2% of genome are coding genes (transcribed into mRNAs), while actually more than 80% of the genome are transcribed actively which means majority of RNAs are noncoding RNAs. A large number of dysregulated noncoding RNAs have been identified in pancreatic cancers(PDA), but only a limited number of them was studied. To explore the importance of the noncoding RNAs (long noncoding RNAs and miRNAs) and maybe some coding genes, we will use NGS-based high throughput screening method combining with gene editing strategy to identify key noncoding RNAs that are essential for pancreatic tumor cell proliferation/apoptosis and further validate their functions in tumor formation in PDA mouse model, illustrating the under