Endocytosis is a critical biological process for cell growth and viability. It transports macromolecules that cannot pass through the membrane passively. Endocytosis also plays important roles in the delivery of medicines for therapeutic treatments. Over the last decades, a lot of research has been focused on developing advanced imaging methods to monitor endocytosis events in living cells and tissues. We aim to use fluorescence methods to study endocytosis of proteins, directly monitoring the molecular machinery of endocytic pathways in liver cells. The utilization of fluorescent proteins is advantageous due to their high sensitivity and ability to be detected even at low intensities. Additionally, fluorescence exhibits remarkable resistance against interference, making it an ideal tool for precise localization visualization purposes. Through this study, students will be able to receive basic biochemical training, and to be exposed to frontier research in the field of targeted biomedicine delivery.