Description
Cancer has been extensively studied to develop better therapeutics, however current treatments are still considered to be less-effective, leading to the poor prognosis. Recently, DNA vaccine has been one of promising cancer treatments by inducing systemic anti-tumor immune responses with little side effects. However, DNA vaccine should overcome some barriers for the clinical applications that requires effective DNA delivery to cell nuclei and cell/tissue-specific targeting. Here, we aim to examine a DNA vaccine delivery system using cell penetration peptide-displaying chromatin. In this study, the histone genes were engineered to fuse either Trans-activator of transcription (TAT) peptide derived from Human immunodeficiency virus (HIV) or fusogenic region of glycoprotein H peptide from Herpes simplex virus (HSV) at the N terminus. Individual histones and octamers were prepared for chromatin assembly in vitro using plasmids. Our results showed high penetration efficiency of HSV gH peptide-fused histones and possible nuclear localization evidence of gH modified chromatins. The studies suggest that chromatin can be a useful platform for a potential DNA vaccine delivery application, which deem to explore further tumor antigen gene expressions in tissue culture models and animal studies.| Period | 1 Sept 2021 → 31 May 2023 |
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| Examinee | Tatsuhiko Kadowaki & Jeong Park |
| Degree of Recognition | International |
Keywords
- DNA vaccine delivery