Stenotrophomonas maltophilia is an opportunistic pathogen that causes infections in immunocompromised patients. Understanding its genetic diversity and transmission patterns is crucial for improving clinical outcomes. This project aims to analyze the whole-genome sequencing data of 117 clinical isolates of S. maltophilia and integrate publicly available genomic sequences and metadata. By constructing a national and global transmission model, we will track the spread and evolution of this pathogen. The findings will provide insights into antimicrobial resistance mechanisms and potential outbreak sources, contributing to better infection control strategies. Through this research, students will gain hands-on experience in bioinformatics, epidemiology, and microbial genomics, fostering essential skills for careers in microbiology and public health.